Functional Food Chemistry Lab. -

Functional Food Chemistry Laboratory

Food, Nutrition, and Health

Food Science and Nutrition Major

Professor at Pukyong National University (2018-Present) Postdoctoral Researcher (North Carolina A&T State University: 2015-2017) Ph.D.: Nutrition Science (University of Connecticut: 2010-2015) M.S.: Food Science and Biotechnology (KyungHee University: 2007-2009) B.S.: Food Science and Biotechnology (KyungHee University: 2000-2007)
Sang Gil Lee: Food Science and Nutrition Major

백수현

박사

Natural compounds analysis (농축산물 잔류농약분석 및 해조류 생리활성물질 분석)

이주원

석박사

Natural compounds analysis (해조류 생리활성물질 및 중금속 분석)

이혜주

석박사

Functional food research

정승진

박사

Functional food research

박채원

석사

Functional Food Research

안현우

석사

Food processing (3D food printer 잉크 소재 개발)

조하란

학사연구원

Food processing (3D food printer 잉크 소재 개발)

한나라

학부연구생

Food processing (3D food printer 잉크 소재 개발)

정은선

연구원

연구지원

강경옥

박사 후 연구원

기능성 식품, 영양 평가 연구

박예나

석사

한국식품연구원

윤현정

석사

한국식품연구원

서찬

연구교수

국립호남권생물자원관

백수현

석사

부산지방식품의약품안전청

Screening of antioxidant capacity of Nepali medicinal plants with a novel singlet oxygen scavenging assay, Food Science and Biotechnology

2022. 10. 2

Sang Gil Lee Chan Seo, Hyun Woo An, Won Han, Joo Won Lee, Krishna K. Shrestha, Won-Kyo Jung, Joong Ho Shin

Examination of Microcystin Adsorption by the Type of Plastic Materials Used during the Procedure of Microcystin Analysis, Toxins

2022. 7. 9

Chan Seo, Joo Won Lee, Won-Kyo Jung, Yoon-Mi Lee, Seungjun Lee, Sang Gil Lee Publication date

Effects of black chokeberry on cholesterol metabolism in HepG2 cells, Korean Journal of Food Science and Technology

2022. 6. 8

Sang Gil Lee, Bohkyung Kim

Nutraceutical potential of polyphenol-rich Sargassum species grown off the Korean coast: a review, Food Science and Biotechnology

2022. 3. 19

Chan Seo, Seung Jin Jeong, Hyun Jung Yun, Hye Ju Lee, Joo Won Lee, Hyun Woo An, Nara Han, Won-Kyo Jung, Sang Gil Lee

Phenolic compounds in common buckwheat sprouts: composition, isolation, analysis and bioactivities, Food Science and Biotechnology

2022. 3. 19

Ahmad Rois Mansur, Sang Gil Lee, Bong-Han Lee, Sang Gyu Han, Sung-Won Choi, Won-Jae Song, Tae Gyu Nam

Effects of Various Extraction Factors on Protein Yield of Haliotis discus hannai (Abalone), Applied Sciences

2021. 2. 11

Hyun-Jung Yun, Seung-Jin Jeong, Yoonmi Lee, Hyeung-Rak Kim, Bohkyung Kim, Sanggil Lee

Identification of Selenoprotein H Isoforms and Impact of Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2 Other Selenoproteins in 293T Cells, The Journal of nutrition

2021. 11. 12

Lei Cao, Tibor Pechan, Sanggil Lee, Wen-Hsing Cheng

The Effects of Polyphenol-Rich Black Elderberry on Oxidative Stress and Hepatic Cholesterol Metabolism, Applied Sciences

2021. 10. 16

Sohyeon Jeon, Sanggil Lee, Yeoni Choi, Bohkyung Kim

Effects of UV and Heating on the Stability of Fucoxanthin, Total Phlorotannin and Total Antioxidant Capacities in Saccharina japonica Ethanol Extract and Solvent Fractions, Applied Sciences

2021. 1. 8

Suhyeon Baek, Lei Cao, Hyeju Lee, Yoonmi Lee, Sanggil Lee

Sargahydroquinoic acid (SHQA) suppresses cellular senescence through Akt/mTOR signaling pathway, Experimental Gerontology

2021. 8. 1

Lei Cao, Sang Gil Lee, Sang-Hyug Park, Hyeung-Rak Kim

Optimization of the salt content in fish surimi ink for food 3D Printing, Korean J. Food Sci. Technol

2021. 2. 28

Chae-Hyeon Lee, Myeong-Eun Kim, Yujia Yang, Yu-Jin Son, Ji-A Lee, Eun-Soon Lyu, Un Ju Jung, Beodeul Kang, Sang Gil Lee

Extraction, Identification, and Health Benefits of Anthocyanins in Blackcurrants (Ribes nigrum L.), Applied Sciences

2022. 2. 2

Lei Cao, Yena Park, Sanggil Lee, Dae-Ok Kim

The Comparison of Total Phenolics, Total Antioxidant, and Anti-Tyrosinase Activities of Korean Sargassum Species, Journal of Food Quality

2021. 1. 18

Su Hyeon Baek, Lei Cao, Seung Jin Jeong, Hyeung-Rak Kim, Taek Jeung Nam, Sang Gil Lee

Influence of production systems on phenolic characteristics and antioxidant capacity of highbush blueberry cultivars, Journal of Food Science

2021. 10. 12

Young Sung Jung, In Ae Kwak, Sang Gil Lee, Hye-Sung Cho, Youn-Sup Cho, Dae-Ok Kim

Potential Anti-Aging Substances Derived from Seaweeds, Marine drugs

2020. 11. 18

Lei Cao, Sang Gil Lee, Kwon Taek Lim, Hyeung-Rak Kim

Phenolic Profiles of Hardy Kiwifruits and Their Neuroprotective Effects on PC-12 and SH-SY5Y Cells against Oxidative Stress, Korean Society for Microbiology and Biotechnology

2020. 6. 28

Ha-Ram Jeong, Kwan Joong Kim, Sang Gil Lee, Hye Sung Cho, Youn-Sup Cho, Dae-Ok Kim

Anti-Inflammatory and Antioxidant Effects of Anthocyanins of Trifolium pratense (Red Clover) in Lipopolysaccharide-Stimulated RAW-267.4 Macrophages, Nutrients

2020. 3. 4

Sang Gil Lee, Cindi R Brownmiller, Sun-Ok Lee, Hye Won Kang

Meroterpenoid-Rich Fraction of the Ethanol Extract of Sargassum Serratifolium Suppresses Collagen-Induced Rheumatoid Arthritis in DBA/1J Mice Via Inhibition of Nuclear Factor κB Activation, Molecular nutrition & food research

2020. 2. 12

Eun‐Ji Joung, Misung Kwon, Wi‐Gyeong Gwon, Lei Cao, Sang‐Gil Lee, Tadanobu Utsuki, Nobuko Wakamatsu, Jae‐Il Kim, Hyeung‐Rak Kim

Long-Term Blackcurrant Supplementation Modified Gut Microbiome Profiles in Mice in an Age-Dependent Manner: An Exploratory Study, Nutrients

2020. 1. 21

Lei Cao, Sang Gil Lee, Melissa M Melough, Junichi R Sakaki, Kendra R Maas, Sung I Koo, Ock K Chun

Analysis and comparison of bioactive compounds and total antioxidant capabilities of Korean brown algae, Korean Journal of Food Science and Technology

2020. 2. 6

Chae-Hyeon Lee, Ye Na Park, Sang Gil Lee

Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells, Fisheries and Aquatic Sciences

2019. 12. 12

Wi-Gyeong Gwon, Sang-Gil Lee, Jae-Il Kim, Young-Mog Kim, Seon-Bong Kim, Hyeung-Rak Kim

Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components, Food chemistry

2019. 1. 4

Sujin Lim, An-Hong Choi, Misung Kwon, Eun-Ji Joung, Taisun Shin, Sang-Gil Lee, Nam-Gil Kim, Hyeung-Rak Kim

Change of fucoxanthin and total antioxidant capacities of Saccharina japonica during the drying process, Korean Journal of Food Science and Technology

2019. 10. 4

Su Hyeon Baek, Hye Ju Lee, Chae Hyeon Lee, Taek-Jeong Nam, Sang Gil Lee

Blackcurrant supplementation improves trabecular bone mass in young but not aged mice, Nutrients

2018. 5. 11

Junichi Sakaki, Melissa Melough, Sang Gil Lee, Judy Kalinowski, Sung I Koo, Sun-Kyeong Lee, Ock K Chun

Flavonoids, Potential Bioactive Compounds, and Non-Shivering Thermogenesis, Nutrients

2018. 8. 25

Hye Won Kang, Sang Gil Lee, Dammah Otieno, Kyoungsoo Ha

Real-Time PCR (QuantStudio 1)

실시간으로 PCR 증폭산물의 생성과정을 모니터링하여 target DNA의 양을 측정하는 장비 ( It uses fluorescence-based polymerase chain reaction (PCR) reagents to perform quantitative detection of target nucleic acid sequences. )

JeioTech PCR Workstations(Hood & Clean bench)(PW-01)

공기필터 가동과 UV조사를 통해 캐비닛 내부를 외부의 환경과 분리하여 시료를 오염으로부터 보호하는 기기 The device operates an air filter and irradiates UV to separate the interior of the cabinet from the external environment to protect the sample from contamination.

Biolus Biological Safety Cabinet(Clean bench) (CHC-111A2)

공기필터 가동과 UV조사를 통해 캐비닛 내부를 외부의 환경과 분리하여 시료를 오염으로부터 보호하는 기기 ( It uses filters and controlled airflows to capture contamination and establish a contamination-free culture. )

배양 현미경(Leica DMIL Inverted Microscope)

Cell의 morphology와 Cell Confluence를 확인하여 Cell Condition을 확인 ( It uses a fixed stage with an objective lens for magnification for precise cell viewing and analysis. )

CO2 incubator(Sanyo MCO-17AI)

고온멸균과 HEPA필터를 이용해 오염원을 제거하고 온도, 습도, 산소 제어를 통해 세포 배양 환경을 최적화할 수 있는 기기 ( It copies the cells’ natural environment by controlling physical parameters such as the temperature, humidity, and CO2 content. )

Automated Cell Counter(BIORAD TC20)

Trypan Blue reagent를 이용하여 단위부피당 살아있는 세포의 수를 세어주는 장비 ( It automatically and accuarately counts the number of cells. )

GeneAmp PCR system 9700 Base Module

중합효소 연쇄반응으로 특정DNA를 증폭하는 장비 ( It amplifies DNA using the polymerase chain reaction (PCR) process. )

Gel imaging System (GE ImageQuant LAS 500)

카메라 이미지 분석 시스템으로 DNA, 단백질 등의 Chemiluminescence western blot, 비색, 청색/UV 형광 이미지 분석에 활용되는 기기 ( It is a cooled charge-coupled device imager for imaging of chemiluminescent Western blots, fluorescent protein and DNA gel stains, and white light imaging of colorimetric stains and markers. )

Seahorse XF96 Extracellular Flux Analyzer

Well plate에서 live cell의 산소 소비율(OCR) 및 세포외 산성화율(ECAR)을 실시간으로 측정하여 미토콘드리아 호흡 등의 세포 기능을 측정하는 기기 ( It simultaneously measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells on a well plate in real time to measure cell functions such as mitochondrial respiration. )

Metabolic Cage

In vivo 실험을 위한 animal 들의 환경을 관리 ( It allows measurement of fluid intake, and to separate and collect feces and urine for numerous qualitative and quantitative determinations. )

Mouse Dexa Machine (Medikors)

DXA (Dual energy X-ray Absorptiometry) 방식을 이용하여,실험동물의 골밀도 (BMD, BMC)와 체성분 (FAT, LEAN) 등을측정 및 분석 ( It measures and analyzes bone mineral density (BMD) and body composition (BMC, FAT, LEAN) of experimental animals using DXA (Dual energy X-ray Absorptiometry) method )

단백질 전기영동 장치 (Bio-rad mini protean tetra cell + powerpac hc)

유체를 통해 전기장을 형성하여 전하를 띄는 분자를 크기나 분자량에 따라 분리 및 정제하는 기기 ( This mini-format, vertical polyacrylamide gel electrophoresis system provides rapid, high-resolution separation of protein (or DNA) mixtures by SDS-PAGE or native PAGE. )

Beads Homogenizer Analytik jena

Beads를 사용하여 샘플을 균일하게 분쇄하고, 냉각을 통한 샘플의 균질화를 유지하는 기기 ( It uniformly crushes samples using Beads and maintain homogenization of samples by cooling )

Flow cytometer(Guava Muse - Luminex)

이미지 분석을 기반으로 상대형광 및 유세포 분석을 통해 세포 정량 및 분석하는 기기 ( It uses miniaturized fluorescent detection and microcapillary technology to quantify cell analysis, such as cell viability, phosphorylation, and apoptosis. )

Pierce G2 Fast blotter

Trans buffer를 사용하여 SDS-PAGE gel에 있는 10-300kDa정도의 단백질을 nitrocellulose, PVDF membrane로 옮기는 기기 ( It transfers proteins around 10–300 kDa from polyacrylamide gels to nitrocellulose or PVDF membrane using Trans buffer. )

BioTek Cytation 5 Cell Imaging Multimode Reader

자동현미경 검사와 마이크로 플레이트 감지, 형광 및 발광 감지, 흡광도 감지가 가능하며 세포이미지 및 데이터 캡처에 대한 조작과 분석을 제공하는 기기 ( It combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows.; The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. )

Xevo TQ-MS

시료 내 성분을 이온화하여 질량을 이용해 분리 및 분석하는 기기 ( It ionizes the components in the sample and then separate and analyze them based on mass difference )

Agilent 1100 HPLC - ELSD

유기화합물을 성분 별로 분리 후 시료와 용매를 증기화하여 빛을 산란시켜 물질을 검출하는 기기 ( It separates, identifies, and quantifies individual compounds in a mixture by measuring their evaporative light-scattering )

Dionex Summit HPLC System with UVD170U UV/Vis Detector

유기화합물을 성분 별로 분리하여 함유량을 측정하는 기기 ( It identifies and quantifies organic components in a mixture after separating them )

nitrogen concentrator (Hurricane – Lite )

노즐에 의한 질소기체 분사로 장착된 농축관 내 시료의 증발 용매를 제거하는 기기 ( It removes the evaporation solvent of a sample in the mounted concentration tube by spraying nitrogen gas through a nozzle )

Solid phase extraction system (HSE-08A)

SPE 컬럼에 일정한 유속으로 액체를 주입하여 효율적으로 분석물을 추출 및 정제할 수 있는 기기 ( It efficiently extracts and refines analytes by injecting liquid into the SPE column at a constant flow rate )

Gerhardt Protein and Nitrogen Analysis System

킬달 질소정량법을 응용하여 시료 중에 포함된 질소 함량을 측정하거나 단백질을 정량하는 기구 ( It measures nitrogen content in samples or quantifies proteins by applying the Kildal nitrogen quantification method )

Solvent Extractor (SER 148)

휘발성 용매에 고체 샘플을 침지 및 가열한 후 용매를 증발시키는 과정으로 비휘발성 물질을 추출하는 기기 ( It extracts nonvolatile materials by immersing and heating solid samples in a volatile solvent and evaporating the solvent. )

Colorimeter

표준색을 기준으로 비교치를 측정하여 색차를 통해 색을 측정하는 장비 ( It measures and compares color differences based on the standard color. )

$Digital refractometer$

Digital refractometer

용액의 당도를 퍼센트 (0~50%)와 굴절률(1.3330~1.4200nD)로 측정하여 정확한 당도 값을 측정하는 기기 ( It determines the exact sugar content by measuring the sugar content of a solution in percent (0 to 50%) and the refractive index (1.3330 to 1.4200 nD) )

$Refractometer$

Refractometer

용액 속에 녹아 있는 물질의 양에 따라 빛이 굴절하는 각도의 차이로 수용액의 농도를 측정하는 기기 ( It measures the concentration of the aqueous solution using the difference in the angle of refraction of light depending on the amount of substance dissolved in the solution. )

Food 3D printer (YOLILO)

개인 맞춤형 푸드잉크를 활용하여 목표하는 3D모델을 압출적층방식으로 출력하는 기기 ( It outputs 3D models in an extrusion lamination method uses customized food ink )

Rheometer (Anton paar MCR92)

유체에 연속적인 회전 및 진동을 가하여 물질의 변형 및 거동 평가를 통해 유변물성을 측정하는 기기 ( It measures the rheological properties by applying continuous rotation and vibration to the fluid to evaluate the deformation and behavior of the material )

Texture Analyzer (IMADA FRTS)

여러 프로브를 이용하여 식품의 경도, 점착력, 응집성, 부착성, 탄력성, 씹힘성 등의 텍스쳐를 평가하여 수치화하는 기기 ( It evaluates and quantifies textures such as hardness, adhesion, cohesiveness, adhesion, elasticity, and chewability of food using several probes. )

Freeze dryer (EYELA FDU-2000)

식품을 급속동결함으로써 식품 중의 수분이 미세한 얼음 결정을 형성하도록 하여 진공 중에서 승화시켜 수증기 형태로 수분을 제거하는 기기 ( It rapidly freezes the product and lowers pressure, then removing the ice by sublimation. )

Water Activity Measurement (Novasina Labmaster-aw)

식품 중의 미생물이 이용가능한 자유수인 수분활성도를 측정하여 제품의 손상, 질감의 변화, 산패의 원인 등을 확인할 수 있는 기기 ( It measures the moisture activity, which is the free water available to microorganisms to use for growth in food, which may lead to food spoilage. )

Food 3D PRINTER (smart 3D Foodbot)

개인 맞춤형 푸드잉크를 활용하여 목표하는 3D모델을 압출적층방식으로 출력하는 기기 ( It outputs 3D models in an extrusion lamination method uses customized food ink. )

시브 쉐이커(체 진동기)Sieve Shaker, 체 진탕기(DHSF-U2)

입자를 입도별로 분립할 때 입자의 통과속도를 빠르게 하기 위해 체에 진동을 주는 기기 ( It vibrates the sieve to speed up the passage of particles when separating particles by size. )

에어프라이어(Air fryer)

식품을 가열 및 조리할 수 있는 기기 ( It simulates deep frying without submerging the food in oil. )

Single Screw Extruder

열가소성 원료를 가열하여 녹이고 재료를 압출 및 성형하여 새로운 조직감을 실현할 수 있는 기기 ( It realize a new sense of texture by heating, melting the thermoplastic raw material, and extruding and molding the material. )

Digital Water Bath(JSR JSWB-11T)

수중 히터를 가열하여 수조 내 온도를 일정한 범위로 유지하는 기기 ( It provides constant temperature environment with heated water. )

Compact Digital Microplate Shaker(Thermo Acientific 88880024)

고정한 마이크로플레이트를 설정한 시간 동안 원하는 속도로 일정하게 흔들어 내용물이 교반되도록 하는 기기 ( It is capable of shaking up to four microplates in one run. )

TABLE STEAM STERILIZER(TM-T24J)

-Autoclave- 고온고압 하에서 화학 반응 또는 추출, 살균 등을 하는 내열내압 기기 ( It uses steam under pressure to eliminate living organisms, such as bacteria, viruses, fungi, and spores on items that are placed inside. )

EYELA Water Bath(SB-1000)

-water bath- 수중 히터를 가열하여 수조 내 온도를 일정한 범위로 유지하는 기기 ( They provide constant temperature environment with heated water. )

Rounding Water Bath(C-WBE-B)

-water bath- 수중 히터를 가열하여 수조 내 온도를 일정한 범위로 유지하는 기기 ( They provide constant temperature environment with heated water. )

Sartorius Analytical Lab Balance Scale (CP323S) Max = 320 g, d = 0.001 g

-전자저울- 물체의 질량을 측정하는 기구 ( They measure the mass of an object. )

Sartorius Analytical Balances (ED224S) Max = 220 g, d = 0.1 mg

-전자저울- 물체의 질량을 측정하는 기구 ( They measure the mass of an object. )

HANIL UNION 32R PLUS LARGE CAPACITY REFRIGERATED CENTRIFUGE

-centrifuge- 원심력을 이용하여 성분이나 비중이 다른 액체 또는 액체 내의 고체를 분리 및 정제하는 기구 ( It separates microliter temperature-sensitive heterogeneous mixtures or samples by spinning the samples loaded in a rotor at high speed. )

LABOGENE ScanSpeed 1730R MicroCentrifuge

Thermo Scientific Orion 3-Star benchtop pH meter

-Ph meter - 유리전극과 비교전극 사이에서 발생하는 전위차로부터 pH를 구하는 기구 ( It measures the acidity or alkalinity of a sample to a high degree of accuracy. )

Homogenizer with Remote Digital Controller(HG-15D-Set-B)

-Homogenizer- RPM과 시간을 설정하여 샘플을 기계적으로 균일하게 분쇄하는 기기 ( It mechanically and uniformly pulverizes the sample at a set RPM for a set time. )

Branson Ultrasonic Cleaner (5510R-DTH)

- Sonication - 초음파의 동력적 기능을 이용하여 섞이기 어려운 용액을 섞거나 분산하는 데에 이용되는 기기 ( It uses ultrasonic waves transmitted through liquid to scrub clean the surface of immersed parts. )

Hotplate Stirrer (SciLAB SMSH-20A)

- Stirrer(교반기) - magnetic bar로 자성을 이용하여 고체 및 액체를 교반하거나 가열하는 기기 ( It heats evenly and mixes various types of liquids and solutions. )

Orbital Shaker (KE011-200)

-shaker- 용액이 든 기구를 올려 회전운동을 통해 내용물을 혼합하는 기기 ( It generates a horizontal circular shaking motion to mix liquids in flasks or tubes. )

Vortex Genie (G560E)

- Vortex - 용액을 빠르게 흔들어 균일하게 혼합해주는 기기 ( It mixes small vials of liquid by agitating them. )

DIAPHRAGM VACUUM PUMP (SLVPGM100A-T)

- PUMP - 공기를 흡입하여 대기압보다 낮은 수준의 압력을 생성하는 기기 ( It draws in air and produces a pressure lower than the atmospheric pressure. )

KUKJE SCIEN Electric Muffle Furnace (FU7060)

- 전기로 - 원료를 최대 1100℃로 가열하여 연소 부산물과 시료를 분리 및 가공하는 기기 ( It separates and processes combustion by-products and samples by heating the raw material up to 1100°C. )

부산, 대한민국

실험실에 관한 다양한 문의는 메세지 및 sglee1125@pknu.ac.kr로 이메일 부탁드립니다.